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49 CFR Part 40 Compliant
DOT Drug & Alcohol Testing Services
Mobile, on-site DOT drug testing for FMCSA-regulated motor carriers. Keep your fleet compliant and your drivers on the road with our certified collection services throughout the Bay Area and Middle Georgia.
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SAMHSA-Certified Labs
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DOT-Certified Collectors
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24/7 Post-Accident Response
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Clearinghouse Reporting
Federal DOT Drug Testing Requirements for Motor Carriers
The Department of Transportation (DOT) mandates drug and alcohol testing for all employees performing safety-sensitive functions in transportation industries. Under 49 CFR Part 40 and 49 CFR Part 382, motor carriers must implement comprehensive testing programs to ensure public safety on our roadways.
Non-compliance with DOT drug testing regulations can result in civil penalties up to $16,864 per violation, driver disqualification, and loss of operating authority. QuickCare's mobile testing services help you maintain full compliance while minimizing operational disruption.
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FMCSA Clearinghouse Requirement
Since January 2020, all DOT drug and alcohol violations must be reported to the FMCSA Drug & Alcohol Clearinghouse. Employers must query the Clearinghouse before hiring and conduct annual queries for current drivers. QuickCare handles all required Clearinghouse reporting for our clients.
Full 49 CFR Part 40 Compliance
Our testing protocols strictly adhere to all DOT collection procedures, chain of custody requirements, and MRO review processes as mandated by federal regulations.
Mobile On-Site Collection
Our certified collectors come to your terminal, maintenance facility, or job site—reducing driver downtime by up to 70% compared to off-site clinic visits.
Complete Audit-Ready Documentation
We maintain all required records per DOT retention requirements: 5 years for positive tests and refusals, 1 year for negative tests, and annual MIS reports ready for FMCSA audits.
Critical DOT Testing Deadlines
DOT regulations specify strict timeframes for post-accident testing. Missing these deadlines can result in violations and complicate insurance claims.
8
Hours
Alcohol Testing
Post-accident breath alcohol test must be administered within 8 hours of the accident
32
Hours
Drug Testing
Post-accident urine drug test specimen must be collected within 32 hours
2
Hours
Random Selection
Employees must proceed to collection site within 2 hours of random selection notification
24
Hours
Specimen Shipping
Collected specimens must be shipped to the laboratory within 24 hours of collection
Understanding DOT Drug Testing Regulations
DOT regulations under 49 CFR Part 40 establish standardized procedures for drug and alcohol testing across all DOT-regulated transportation modes.
Six Required Testing Categories
DOT mandates specific testing scenarios throughout the employment lifecycle. Each category has distinct triggers, procedures, and documentation requirements.
- Pre-employment – Before safety-sensitive duties begin
- Random – Unannounced, throughout the year
- Post-accident – Following qualifying accidents
- Reasonable suspicion – Based on trained supervisor observation
- Return-to-duty – After violation, before resuming duties
- Follow-up – Minimum 6 tests in first 12 months post-violation
Covered Safety-Sensitive Employees
FMCSA regulations (49 CFR Part 382) define safety-sensitive functions for commercial motor vehicle operators.
- Commercial drivers requiring a CDL
- Drivers operating vehicles over 26,001 lbs GVWR
- Drivers of vehicles transporting 16+ passengers
- Hazmat drivers requiring placards
- All time waiting to be dispatched
- Vehicle inspection, servicing, and conditioning
DOT 5-Panel Drug Screen
The DOT-mandated drug panel tests for five specific categories of controlled substances as required by SAMHSA guidelines.
- Marijuana (THC) – 50 ng/mL initial, 15 ng/mL confirmation
- Cocaine – Benzoylecgonine metabolite
- Amphetamines – Including methamphetamine, MDMA
- Opioids – Expanded panel includes hydrocodone, oxycodone
- Phencyclidine (PCP)
- Alcohol – Separate EBT testing (0.04 BAC threshold)
DOT Drug Testing Scenarios Explained
Each testing category has specific triggers, procedures, and compliance requirements. Understanding when testing is required helps prevent violations.
| Test Type | When Required | Key Requirements | Timeline |
|---|---|---|---|
|
Pre-Employment
Mandatory
|
Before an employee first performs safety-sensitive functions for any employer | Must receive verified negative result before allowing safety-sensitive work. Must also query FMCSA Clearinghouse. | Result valid for 90 days if no subsequent employer; must be hired within 90 days of collection |
|
Random Testing
Mandatory
|
Unannounced selections throughout the calendar year using scientifically valid random method | 50% of driver pool for drugs, 10% for alcohol annually. Each driver must have equal selection probability. | Driver must proceed to collection site immediately upon notification (within 2 hours) |
|
Post-Accident
Time-Critical
|
After accidents involving: • Fatality (always required) • Bodily injury requiring medical transport AND driver received citation • Disabling vehicle damage AND driver received citation |
Test regardless of fault in fatality accidents. Document if testing cannot be completed within required timeframes. |
Alcohol: Within 8 hours Drugs: Within 32 hours Do not administer after these windows. |
| Reasonable Suspicion | Based on specific, contemporaneous, articulable observations of appearance, behavior, speech, or body odors | Supervisor making determination must have completed DOT-approved reasonable suspicion training. Documentation required. | Test as soon as possible. Alcohol test must be within 8 hours of observation or document why not. |
| Return-to-Duty | After any DOT drug/alcohol violation, before resuming safety-sensitive functions | Must complete SAP evaluation, follow treatment recommendations, and receive verified negative result under direct observation. | No minimum waiting period, but all SAP requirements must be completed first |
| Follow-Up | After returning to duty following a violation, as scheduled by the SAP | Minimum 6 unannounced tests in first 12 months. SAP can extend up to 60 months total. Direct observation required. | As scheduled by SAP; unannounced and unpredictable |
DOT Drug Testing Violation Penalties
Non-compliance with DOT drug and alcohol testing regulations carries significant civil and operational penalties for both carriers and drivers.
$16,864
Per Violation – Standard
Maximum civil penalty for each instance of failing to implement required testing, falsifying records, or allowing untested drivers to operate.
$59,017
Pattern Violations
Maximum penalty for egregious or repeated violations demonstrating a pattern of non-compliance with DOT drug testing regulations.
Disqualification
Driver Consequences
Drivers who test positive, refuse to test, or have violations reported to the Clearinghouse are prohibited from safety-sensitive functions until completing return-to-duty process.
Loss of Authority
Operational Impact
Severe or repeated violations can result in suspension or revocation of operating authority, effectively shutting down carrier operations.
Why Choose QuickCare for DOT Testing
Our mobile DOT drug testing services are designed specifically for the operational realities of the transportation industry.
70% Less Driver Downtime
On-site testing eliminates travel to clinics and waiting room delays. Your drivers spend minutes, not hours, completing required tests—keeping trucks moving and revenue flowing.
24/7 Emergency Response
Accidents don't follow business hours. Our collectors are available around the clock to meet DOT's strict post-accident testing deadlines—8 hours for alcohol, 32 hours for drugs.
Bulletproof Chain of Custody
Our collectors are trained in DOT-specific procedures under 49 CFR Part 40. Every specimen is properly documented, sealed, and transported to ensure legal defensibility of results.
Clearinghouse Integration
We handle required FMCSA Clearinghouse reporting for all violations. Pre-employment queries, annual queries, and violation reporting—fully managed so you stay compliant.
Audit-Ready Records
Complete documentation maintained per DOT retention requirements. When FMCSA conducts a compliance review, your records will be organized, complete, and readily accessible.
Consortium/C-TPA Services
Small fleets can join our consortium for cost-effective random testing pool management. We handle selection, scheduling, and compliance documentation so you can focus on operations.
The Mobile DOT Testing Advantage
Traditional DOT testing forces drivers to leave your facility, travel to a clinic, wait for their turn, and travel back—often consuming 2-4 hours of productive time per test. Our mobile collection service eliminates this operational drain.
Our fully-equipped mobile collection units arrive at your location with everything needed for DOT-compliant testing: certified collectors trained in 49 CFR Part 40 procedures, proper custody and control forms (CCFs), NHTSA-approved Evidential Breath Testing devices, and secure specimen transport supplies.
We coordinate testing around your operational schedule—early morning before dispatch, during driver breaks, or at shift changes—minimizing disruption while ensuring you meet all DOT random testing rate requirements.
70%
Average reduction in driver downtime vs. clinic visits
99.8%
Chain of custody accuracy rate maintained
24/7
Emergency post-accident response availability
Our DOT Testing Process
Every step follows strict 49 CFR Part 40 protocols to ensure specimen integrity, legal defensibility, and full regulatory compliance.
1
Program Setup & Consultation
We review your operation's specific needs: fleet size, driver count, operational schedule, and existing policies. We'll ensure your written drug and alcohol policy meets DOT requirements and establish you as a registered employer in the FMCSA Clearinghouse.
2
Random Selection Pool Management
Using a scientifically valid random selection method, we manage your testing pool to ensure you meet DOT's 50% annual drug testing rate and 10% alcohol rate. Selections are spread throughout the year with unpredictable timing to maintain the deterrent effect.
3
On-Site Specimen Collection
Our DOT-qualified collectors arrive at your facility with all required supplies. We verify driver identity using photo ID, complete the Federal Drug Testing Custody and Control Form (CCF), collect the split-specimen urine sample, and conduct breath alcohol testing using NHTSA-approved EBT devices when required.
4
Laboratory Analysis
Specimens are shipped within 24 hours to a SAMHSA-certified laboratory. Initial immunoassay screening identifies presumptive positives, which undergo confirmation testing using gas chromatography-mass spectrometry (GC/MS) for definitive results.
5
Medical Review Officer (MRO) Review
All non-negative results are reviewed by a licensed physician qualified as a Medical Review Officer. The MRO contacts the donor to discuss any legitimate medical explanations (such as valid prescriptions) before making a final determination. This critical step prevents false positives from valid medications.
6
Results & Clearinghouse Reporting
Results are reported to your Designated Employer Representative (DER) through our secure portal. For any violations, we handle required FMCSA Clearinghouse reporting and provide guidance on next steps including SAP referrals. All documentation is maintained per DOT retention requirements.
DOT Testing Service Areas
QuickCare provides mobile DOT drug testing services throughout the San Francisco Bay Area and Middle Georgia region.
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Primary Service Area
San Francisco Bay Area, California
Comprehensive mobile DOT testing coverage across all nine Bay Area counties. Same-day response available for post-accident testing. Ideal for trucking terminals, distribution centers, and transportation companies along major freight corridors including I-80, I-880, and I-580.
Alameda County
Contra Costa County
Marin County
Napa County
San Francisco
San Mateo County
Santa Clara County
Solano County
Sonoma County
View Bay Area Service Map
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Middle Georgia Region
Mobile DOT testing services covering the Macon metropolitan area and surrounding counties. Strategic coverage along I-75 and I-16 corridors serving trucking operations, logistics companies, and distribution facilities throughout central Georgia.
Bibb County
Houston County
Peach County
Crawford County
Monroe County
Jones County
View Georgia Service Areas
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DOT Drug Testing Pricing
Transparent, competitive pricing for DOT-compliant drug and alcohol testing services. Volume discounts available for fleets with 10+ drivers.
DOT 5-Panel Lab Test
Complete urine drug screen with MRO review
$89.00per test
- DOT 5-panel drug screen (THC, Cocaine, Amphetamines, Opioids, PCP)
- SAMHSA-certified laboratory analysis
- Medical Review Officer (MRO) review included
- Split specimen collection per 49 CFR Part 40
- Federal CCF documentation
- Secure electronic results reporting
DOT Breath Alcohol Test
Evidential Breath Testing (EBT) for alcohol
$64.00per test
- NHTSA-approved EBT device
- DOT-qualified Breath Alcohol Technician (BAT)
- Immediate screening results
- Confirmation test for results ≥0.02 BAC
- Alcohol Testing Form (ATF) documentation
- Compliant 15-minute waiting period observed
Random Testing Program
Complete C/TPA consortium management
Custompricing
- Consortium/Third-Party Administrator services
- Scientifically valid random selection
- Pool management & rate compliance
- Selection notification coordination
- Annual MIS report preparation
- FMCSA Clearinghouse query management
DOT Drug Testing FAQs
Common questions about DOT drug and alcohol testing requirements, procedures, and our mobile testing services.
DOT drug testing follows strict federal guidelines under 49 CFR Part 40 that differ significantly from standard workplace drug testing:
- Standardized 5-panel test – DOT mandates specific drugs and cutoff levels; employers cannot modify the panel
- Split-specimen collection – All DOT tests require collection of two specimens (primary and split) for potential re-testing
- SAMHSA-certified laboratories only – Results from non-certified labs are not acceptable
- Mandatory MRO review – A licensed Medical Review Officer must review all non-negative results
- Federal CCF forms – DOT-specific Custody and Control Forms are required; employer forms are not acceptable
- Strict chain of custody – Detailed procedures for specimen handling, sealing, and transport
- Clearinghouse reporting – All violations must be reported to the FMCSA Drug & Alcohol Clearinghouse
Non-DOT testing allows employers more flexibility in panel selection, collection methods, and result handling. However, DOT-regulated employers must maintain completely separate testing programs—DOT and non-DOT tests cannot be combined or substituted.
FMCSA requires motor carriers to randomly test their safety-sensitive employees at specific annual rates:
- Drug Testing: Minimum 50% of the average number of driver positions annually
- Alcohol Testing: Minimum 10% of the average number of driver positions annually
Important compliance requirements:
- Tests must be spread reasonably throughout the year—you cannot batch all tests in one quarter
- Selection must use a scientifically valid random method where each driver has an equal chance of selection
- Drivers can be selected multiple times per year—previous selection does not exclude them from future draws
- Upon notification, drivers must proceed to the collection site immediately (within 2 hours maximum)
- The selection process itself must be documented and auditable
QuickCare's consortium services handle all aspects of random selection and scheduling, ensuring you meet these requirements without administrative burden.
A verified positive DOT drug test triggers a mandatory sequence of events:
- Immediate Removal: The driver must be immediately removed from all safety-sensitive functions. They cannot operate a commercial vehicle.
- Clearinghouse Reporting: The employer (or C/TPA) must report the violation to the FMCSA Drug & Alcohol Clearinghouse within 3 business days.
- SAP Referral: The employer must provide the driver with a list of qualified Substance Abuse Professionals (SAPs) and inform them of the return-to-duty process.
- SAP Evaluation: The driver must complete an initial evaluation with a DOT-qualified SAP, who will recommend education and/or treatment.
- Treatment Completion: The driver must complete all SAP-recommended treatment before proceeding.
- SAP Follow-Up: After treatment, the SAP conducts a follow-up evaluation to determine if the driver can return to safety-sensitive duties.
- Return-to-Duty Test: Before resuming duties, the driver must pass a return-to-duty drug test with a verified negative result. This test is conducted under direct observation.
- Follow-Up Testing: The SAP prescribes a follow-up testing plan—minimum 6 unannounced tests in the first 12 months, potentially extending up to 60 months.
Note: Employers are not required to hold the driver's position during this process, though some choose to do so. The entire return-to-duty process is at the driver's expense unless the employer's policy states otherwise.
DOT post-accident testing is required in specific circumstances—not every accident triggers testing requirements:
Testing is ALWAYS required if:
- The accident resulted in a fatality (regardless of who was at fault or who received a citation)
Testing is required if the driver received a citation AND:
- Bodily injury to any person who required immediate medical treatment away from the scene, OR
- One or more vehicles incurred disabling damage requiring tow-away
Critical Timing Requirements:
- Alcohol: Test must be administered within 8 hours of the accident
- Drugs: Specimen must be collected within 32 hours of the accident
If testing cannot be completed within these windows, you must document the reasons and cease attempts. The driver should not consume alcohol for 8 hours following the accident or until tested.
QuickCare offers 24/7 emergency response for post-accident testing throughout our service areas to help you meet these strict deadlines.
The DOT 5-panel drug test screens for the following substance categories:
- Marijuana (THC) – Initial screen: 50 ng/mL; Confirmation: 15 ng/mL. Note: Even in states where marijuana is legal, it remains prohibited for DOT safety-sensitive employees.
- Cocaine – Tests for the benzoylecgonine metabolite. Initial: 150 ng/mL; Confirmation: 100 ng/mL
- Amphetamines – Including amphetamine, methamphetamine, MDMA (Ecstasy), and MDA. Initial: 500 ng/mL; Confirmation: 250 ng/mL
- Opioids – The expanded panel (since 2018) includes:
- Codeine, Morphine, Heroin (6-AM)
- Hydrocodone, Hydromorphone
- Oxycodone, Oxymorphone
- Phencyclidine (PCP) – Initial: 25 ng/mL; Confirmation: 25 ng/mL
Alcohol Testing: Conducted separately using Evidential Breath Testing (EBT) devices. Results of 0.02-0.039 BAC require removal from safety-sensitive duties for 24 hours. Results of 0.04 BAC or higher constitute a DOT violation.
Important: Employers cannot add substances to the DOT panel or change cutoff levels. Additional non-DOT testing must be conducted as a completely separate test.
Yes, QuickCare provides 24/7 emergency response for post-accident and reasonable suspicion testing throughout our California Bay Area and Middle Georgia service regions.
Our emergency response capabilities include:
- Dedicated emergency dispatch line for after-hours testing requests
- Mobile collectors on-call who can respond to your location, accident scenes, or hospitals
- Complete testing equipment including EBT devices and urine collection supplies
- Rapid response times to meet DOT's 8-hour alcohol and 32-hour drug testing deadlines
- Coordination with law enforcement and medical personnel when necessary
For established clients, we recommend programming our emergency line into your accident response protocols and training dispatchers on when DOT post-accident testing is required. This preparation ensures you can initiate testing immediately when an accident occurs.
The FMCSA Drug & Alcohol Clearinghouse is a secure federal database that tracks drug and alcohol violations for commercial driver's license (CDL) holders. Operating since January 2020, it serves as a central repository for violation information.
Employer Requirements:
- Pre-employment query – Must query the Clearinghouse before allowing any driver to perform safety-sensitive functions
- Annual query – Must conduct a query at least once per year for each currently employed driver
- Violation reporting – Must report positive tests, refusals, and actual knowledge violations within 3 business days
- Return-to-duty reporting – Must report when a driver completes the return-to-duty process
Query Types:
- Limited query – Reveals whether a driver has any violations (requires driver consent)
- Full query – Reveals complete violation details (requires electronic driver consent in the Clearinghouse)
QuickCare handles Clearinghouse reporting for all violations identified through our testing services and can assist with query management as part of our consortium services.
Simplify Your DOT Compliance
Partner with QuickCare for mobile DOT drug testing that keeps your drivers on the road and your company in full federal compliance. Schedule a consultation to discuss your fleet's needs.